Solution Study of the NF-κB p50–DNA Complex by UV Laser Protein–DNA Cross-linking¶

Dimitar Angelov; Monique Charra; Christoph W. Müller; Jean Cadet; Stefan Dimitrov

doi:10.1562/0031-8655(2003)077<0592:SSOTNP>2.0.CO;2

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Dimitar Angelov,^1,2,* Monique Charra,^1,2 Christoph W. Müller,³ Jean Cadet,^** Stefan Dimitrov^1,2

¹aLaboratoire de Biologie Moléculaire et Cellulaire de la Différenciation, INSERM Institut Albert Bon
²bEuropean Molecular Biology Laboratory, Grenoble Outstation, Grenoble Cedex, France
³cCEA/Département de Recherche Fondamentale sur la Matière Condensée, SCIB/LAN and FRE 2600, Grenoble

^*†Current address: Institute of Solid State Physics, Bulgarian Academy of Sciences, Sofia, Bulgaria
^***To whom correspondence should be addressed at: Département de Recherche Fondamentale sur la Matiére Condensée, SCIB/LAN, CEA/Grenoble, Grenoble, Cedex 9 F-38054, France. Fax: 334-3878-5090; cadet@drfmc.ceng.cea.fr

Abstract

In this study, we describe a new approach for studying protein–DNA interactions in solution. The approach is based on mapping the UV laser–induced protein–DNA cross-links between the amino acids of the protein and the DNA bases that are in direct contact. The approach was applied for studying the solution structure of the human necrosis factor (NF)–κB p50 homodimer bound to a 37 base pair DNA. Several points of contact identical to those observed in the NF-κB–DNA crystal structure were found between the two biomolecules. Evidence is provided for the occurrence of two new contact points, one for each DNA strand. These new points of contact are located symmetrically a base apart from the extremity of the binding sequence.

Citation Download Citation

Dimitar Angelov, Monique Charra, Christoph W. Müller, Jean Cadet, and Stefan Dimitrov "Solution Study of the NF-κB p50–DNA Complex by UV Laser Protein–DNA Cross-linking," Photochemistry and Photobiology 77(6), 592-596, (1 June 2003). https://doi.org/10.1562/0031-8655(2003)077<0592:SSOTNP>2.0.CO;2

Received: 15 January 2003; Accepted: 1 March 2003; Published: 1 June 2003

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